Impact of sperm genome decay on Day-3 embryo chromosomal abnormalities from advanced-maternal-age patients Infertile male patients often exhibit unconventional semen parameters, including DNA fragmentation, chromatin dispersion, and aneuploidy-collectively referred to as sperm genome decay (SGD). We investigated the correlation of SGD to embryo chromosomal abnormalities and its effect on clinical pregnancy rates in patients with …
Effect of semen preparation technique and its incubation on sperm quality in the Moroccan population In in vitro fertilisation (IVF), sperm preparation as critical part and influencing the sperm quality is especially dependent on the chosen technique itself and incubation parameters including temperature and CO2. In this study, we compared firstly density-gradient centrifugation technique (DGC) …
Maternal age is a significant factor influencing in vitro fertilization (IVF) outcomes. Oxidative
stress (OS) is one of the major causes of age-related cellular and molecular damage. The purpose
of this work was to investigate the correlation between maternal age with intrafollicular antioxidants
and OS markers in follicular fluid (FF), and also to determine the OS status in patients
of advanced age.
Luteal estradiol pretreatment (LEP) to IVF protocols designed to improve follicle synchronization and
retrieval of mature oocytes. We conducted a retrospective study including women undergoing IVF program
who were given a course of 4mg oral estradiol-17b daily from day 20 of the same cycle until day 1
of their next cycle before starting an antagonist protocol…
Circulating anti-m€ullerian hormone (AMH) and antral follicle count (AFC) are the best predictors of IVF
outcomes. However, in extreme low AMH range especially for young patients, AMH prediction power
loose its specificity to give real idea of pregnancy chance with IVF treatments and good prognosis of an
extremely reduced ovarian reserve and expected poor response.
Intrauterine insemination of cultured peripheral blood mononuclear cells prior to embryo transfer improves clinical outcome for patients with repeated implantation failures Implantation failure is a major limiting factor in assisted reproduction improvement. Dysfunction of embryo–maternal immuno-tolerance pathways may be responsible for repeated implantation failures. This fact is supported by immunotropic theory stipulating that maternal immune …
Human in vitro maturation (IVM) is an approach that
was developed about 50 years ago, although its use
in IVF methods is still limited mainly to oncofertility
treatments and female factor infertility, especially in the case
of polycystic ovary syndrome (PCOS).
Alcohol intake and cigarette smoking are the major lifestyle factors with negative impact
on fertility. We were interested to evaluate the negative impact of these factors
on oxidative stress (OS), enzymatic antioxidant activity (EAO) of spermatozoa and on
its DNA damage.